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retroviral plpcx fasn flag vector  (Addgene inc)


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    Structured Review

    Addgene inc retroviral plpcx fasn flag vector
    Retroviral Plpcx Fasn Flag Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/retroviral+vector+plpcx/FASN+(Plasmid+%2338793)/pm39971971-339-12-34
    Average 91 stars, based on 1 article reviews
    retroviral plpcx fasn flag vector - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Amplification:

    Article Title: LncRNA HOTAIR recruits SNAIL to inhibit the transcription of HNF4α and promote the viability, migration, invasion and EMT of colorectal cancer
    Article Snippet: Target of sh-HOTAIR or sh-SNAIL were picked using BLOCK-iT TM RNAi Designer ( http://rnaidesigner.thermofisher.com/rnaiexpress/ ). .. Sh-NC, sh-HOTAIR, sh-SNAIL (ordered from Invitrogen, Thermo Fisher Scientific) and HNF4α cDNA (amplified by PCR) were inserted in retroviral vector pLPCX (Addgene) to obtain pLPCX-shNC, pLPCX-shHOTAIR, pLPCX-shSNAIL and pLPCX- HNF4α. ..

    Polymerase Chain Reaction:

    Article Title: LncRNA HOTAIR recruits SNAIL to inhibit the transcription of HNF4α and promote the viability, migration, invasion and EMT of colorectal cancer
    Article Snippet: Target of sh-HOTAIR or sh-SNAIL were picked using BLOCK-iT TM RNAi Designer ( http://rnaidesigner.thermofisher.com/rnaiexpress/ ). .. Sh-NC, sh-HOTAIR, sh-SNAIL (ordered from Invitrogen, Thermo Fisher Scientific) and HNF4α cDNA (amplified by PCR) were inserted in retroviral vector pLPCX (Addgene) to obtain pLPCX-shNC, pLPCX-shHOTAIR, pLPCX-shSNAIL and pLPCX- HNF4α. ..

    Retroviral:

    Article Title: LncRNA HOTAIR recruits SNAIL to inhibit the transcription of HNF4α and promote the viability, migration, invasion and EMT of colorectal cancer
    Article Snippet: Target of sh-HOTAIR or sh-SNAIL were picked using BLOCK-iT TM RNAi Designer ( http://rnaidesigner.thermofisher.com/rnaiexpress/ ). .. Sh-NC, sh-HOTAIR, sh-SNAIL (ordered from Invitrogen, Thermo Fisher Scientific) and HNF4α cDNA (amplified by PCR) were inserted in retroviral vector pLPCX (Addgene) to obtain pLPCX-shNC, pLPCX-shHOTAIR, pLPCX-shSNAIL and pLPCX- HNF4α. ..

    Plasmid Preparation:

    Article Title: LncRNA HOTAIR recruits SNAIL to inhibit the transcription of HNF4α and promote the viability, migration, invasion and EMT of colorectal cancer
    Article Snippet: Target of sh-HOTAIR or sh-SNAIL were picked using BLOCK-iT TM RNAi Designer ( http://rnaidesigner.thermofisher.com/rnaiexpress/ ). .. Sh-NC, sh-HOTAIR, sh-SNAIL (ordered from Invitrogen, Thermo Fisher Scientific) and HNF4α cDNA (amplified by PCR) were inserted in retroviral vector pLPCX (Addgene) to obtain pLPCX-shNC, pLPCX-shHOTAIR, pLPCX-shSNAIL and pLPCX- HNF4α. ..



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    NR4A1 overexpression in mammary epithelial cells . MCF-10A cells were infected either with a <t>retroviral</t> construct containing the NR4A1 cDNA or with an empty vector control. Overexpression was confirmed at the mRNA level by (a) RT-PCR, and at the protein level by (b) western blot and (c) immunofluorescence.
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    NR4A1 overexpression in mammary epithelial cells . MCF-10A cells were infected either with a retroviral construct containing the NR4A1 cDNA or with an empty vector control. Overexpression was confirmed at the mRNA level by (a) RT-PCR, and at the protein level by (b) western blot and (c) immunofluorescence.

    Journal: Breast Cancer Research : BCR

    Article Title: Dissecting the transcriptional networks underlying breast cancer: NR4A1 reduces the migration of normal and breast cancer cell lines

    doi: 10.1186/bcr2610

    Figure Lengend Snippet: NR4A1 overexpression in mammary epithelial cells . MCF-10A cells were infected either with a retroviral construct containing the NR4A1 cDNA or with an empty vector control. Overexpression was confirmed at the mRNA level by (a) RT-PCR, and at the protein level by (b) western blot and (c) immunofluorescence.

    Article Snippet: The full-length human NR4A1 ORF (Geneservice, Cambridge, UK) was cloned into the Xho I/ Not I sites of the retroviral pLPCX vector (Clontech, Saint-Germain-en-Laye, France).

    Techniques: Over Expression, Infection, Construct, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Western Blot, Immunofluorescence